|Year : 2017 | Volume
| Issue : 1 | Page : 1-9
Role of oxidative stress in liver cancer
Hossein Forouzandeh1, Heibatullah Kalantari2, Najmaldin Saki3, Zahra Foruozandeh4, Ehsan Arefian5, Abbas Farahani6, Ghasem Hassani7, Mohammad Rafi Bazrafshan8, Shima Rasouli9
1 Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz; Gerash Cellular and Molecular Research Center, Gerash University of Medical Sciences, Gerash, Iran
2 Department of Pharmacology and Toxicology, Pharmacy School, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
3 Department of Hematology, Paramedical School, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
4 Gerash Cellular and Molecular Research Center, Gerash University of Medical Sciences, Gerash, Iran
5 Department of Microbiology, School of Biology, College of Science, University of Tehran, Tehran, Iran
6 Student Research Committee, Ahvaz Jundishapur University of Medical Sciences; Department of Microbiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
7 Department of Environmental Health Engineering, Faculty of Public Health, Yasuj University of Medical Sciences, Yasuj, Iran
8 Department of Medical Surgical Nursing, Larestan School of Medical Sciences, Larestan, Iran
9 Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
|Date of Web Publication||29-Jun-2017|
Department of Microbiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz
Source of Support: None, Conflict of Interest: None
The present article provides an overview of the role of oxidative stress in the development and progression of liver cancer (LC). Oxidative stress ensues when the balance between the production of reactive oxygen species (ROS) and reactive nitrogen species overrides the antioxidant defense of the target cell and body fails in detoxifying their harmful effects. Therefore, the interaction of these reactive species with critical cellular macromolecules may cause oxidative damage. Moreover, ROS may interact with cellular components including proteins, lipids, and DNAs, which results in altered target cell function. The accumulation of oxidative damage products has been implicated in both acute and chronic cell injury suggesting a possible role in the pathogenesis of Parkinson's disease, Alzheimer's disease, atherosclerosis, heart failure, myocardial infarction, and cancers. Alcoholism, viral agents, obesity, and smoking increase the occurrence of oxidative stress and consequently the risk of LC.
Keywords: Cancer, carcinogenesis, free radical, liver, oxidative stress
|How to cite this article:|
Forouzandeh H, Kalantari H, Saki N, Foruozandeh Z, Arefian E, Farahani A, Hassani G, Bazrafshan MR, Rasouli S. Role of oxidative stress in liver cancer. Clin Cancer Investig J 2017;6:1-9
|How to cite this URL:|
Forouzandeh H, Kalantari H, Saki N, Foruozandeh Z, Arefian E, Farahani A, Hassani G, Bazrafshan MR, Rasouli S. Role of oxidative stress in liver cancer. Clin Cancer Investig J [serial online] 2017 [cited 2021 May 12];6:1-9. Available from: https://www.ccij-online.org/text.asp?2017/6/1/1/209152
| Introduction|| |
Oxidative stress occurs through overproduction of several types of reactive species in the body or as a result of a decrease in their detoxification mechanisms. These species are called prooxidants including reactive oxygen species (ROS) and reactive nitrogen species (RNS). There are many natural sources of oxidative stress, for example, exposure to environmental oxidants, toxins such as heavy metals, ionizing and ultraviolet irradiation, heat shock, and inflammation. High levels of ROS and RNS exert a toxic effect on intracellular and extracellular macromolecules (e.g., DNA, proteins, and lipid membrane), thus leading to the oxidative damage in different parts of cell.
ROS are chemically reactive components containing oxygen which are a natural by-product of aerobic metabolism cycle. ROS can be supplied through endogenous and/or exogenous resources. Exogenous ROS can be produced either by direct or indirect mechanisms in confronting with drugs, hormones, and other xenobiotic chemicals.,
ROS include a number of species such as superoxide anion (O2−), hydroxyl, and peroxyl radicals and certain nonradicals such as singlet oxygen and hydrogen peroxide (H2O2) that can be easily converted into radicals. Some species including O2− and H2O2 are constantly produced during metabolic processes in all living cells. ROS can be regarded as a trigger of genetic mutations as well as chromosomal alterations, thus contributing to cancer development through various steps of carcinogenesis. In physiological conditions, cellular ROS production is counterbalanced by the action of antioxidant enzymes and other redox molecules. The balance between O2− production and elimination is important for maintaining proper cellular redox state. A moderate increase in ROS can stimulate cell growth and proliferation.,
Besides their harmful effects in clinical conditions, the importance of ROS and RNS as mediators in different cellular processes and cell signaling pathways is apparent., Similarly, RNS include reactive species such as peroxynitrite, nitrogen dioxide, and nitric oxide (•NO). Like ROS, RNS are derived from the interactions of biologically generated free reactive species to form more persistent species resulting in multiple biological effects.,, Because of their potential harmful effects, excessive ROS and RNS must be eliminated quickly from the cell's environment. Antioxidants are the first line of defense against free radical damage and are critical for maintaining optimum health. The body has developed several antioxidant systems to deal with the overproduction of pro-oxidants. These systems can be divided into enzymatic and nonenzymatic. The enzymatic system includes O2− dismutase, catalase, glutathione (GSH) peroxidase, and GSH reductase. None-enzymatic system is being provided by nutrient-derived antioxidants including minerals (Se, Mn, Cu, and Zn), vitamins (A, C, and E), and other compounds (GSH, flavonoids, bilirubin, uric acid, etc.)., Alternatively, oxidative stress occurs in concurrent with the shortage of antioxidant reservoir of the cell. Antioxidant levels provided by either enzymatic or none-enzymatic systems can be decreased through several mechanisms including modification in gene expression, a decrease in their uptake through nutrition, or overproduction of ROS in the cells.,
Acute oxidative injury as a result of above-mentioned events may produce selective cell death and eventually a compensatory increase in cell proliferation. This stimulus may result in the formation of new preneoplastic cells. Similarly, fatal acute oxidative injury may produce unrepaired DNA damage, formation of new mutations and potentially, newly engendered cells. In contrast, the sustained chronic oxidative injury may lead to normal cellular growth under the control of nonfatal modification mechanisms. Moreover, the role of reactive species in the etiology of cancer is supported by epidemiologic studies. These epidemiologic studies specifically illustrated the protective role for antioxidants against cancer development.,
Numerous studies on the liver carcinogens showed a dose-dependent decrease in liver antioxidant concentrations along with an increase in ROS formation and oxidative damage. This increase in oxidative stress correlated with an increase in hepatocytes DNA synthesis.
The liver is one of the largest organs in the human body and the major site for metabolism and excretion. It has a wide range of functions, including detoxification, protein synthesis, and production of pivotal biochemicals necessary for digestion cycle. Liver diseases have become one of the major causes of morbidity and mortality of human beings worldwide, and liver cancers (LCs) seem to be the worst. To an extent, LC ranks fifth in frequency worldwide.
Liver can be affected by primary LC, which primarily arises in the liver, or can emerge following metastasis of cancer cells from other parts of the body to the liver. Because the liver is made up of several different types of cells, several types of tumors can form there. Hepatocellular carcinoma (HCC) is among the most common primary LCs, which is characterized by hepatocytes involvement. Other types of cancers formed within the other structures of the liver include hepatoblastoma (formed by immature liver cells), cholangiocarcinoma (bile duct involvement), angiosarcoma (characterized by blood vessel cells involvement), and fibrosarcoma (connective tissue involvement).,
| The Most Important Organelles Attributed to Reactive Oxygen Species Production|| |
Peroxisomes and reactive oxygen species
Oxygen is consumed in various metabolic cycles in different parts of cell, where mitochondria, endoplasmic reticulum, and peroxisomes are on the top of these sites. Peroxisomes are involved in a variety of important cellular functions, and its major role is considered to be in the decomposition of H2O2.
Peroxisomes play a key role in both the production and scavenging of ROS within the cells. To maintain the equilibrium equivalence between production and scavenging of ROS, peroxisomes harbor several powerful defense mechanisms and antioxidant enzymes. Such conditions are considered to generate peroxisome-induced oxidative stress, which may overwhelm the antioxidant capacity leading to cancer. Furthermore, transition metal ions such as iron and copper are abundant in peroxisomes, and under certain conditions, these metal ions can be released and catalyze the formation of •OH in the Fenton reaction, thus leading to lipid peroxidation, damage of the peroxisomal membrane, and loss of peroxisomal functions.,
Peroxisomal enzymes responsible for reactive oxygen species generation
As shown in [Table 1], peroxisomal enzymes attributed to ROS generation.
|Table 1: Peroxisomal enzymes attributed to reactive oxygen species generation|
Click here to view
Peroxisomal enzymes scavenging reactive oxygen species
Peroxisomal enzymes that scavenge ROS are discussed in [Table 2].
Peroxisome proliferation and induction of oxidative stress
The disproportionate increase of H2O2-generating oxidases is suggested to be responsible for oxidative stress leading to the development of hepatic tumors in rodents treated with peroxisome proliferating compounds. Some compounds have been regarded as peroxisome proliferation stimulants including hypolipidemic drugs, industrial chemicals (e.g., plasticizers, lubricants, and agrochemicals), and other toxic environmental pollutants., However, the oxidative stress does not seem to be exclusively responsible for the development of tumors in rodents exposed to peroxisome proliferators. indeed, other mechanisms such as suppression of apoptosis, perturbation of cell proliferation, and release of O2− radicals from Kupffer cells  have also been suggested to play critical roles in the pathogenesis of tumors associated with peroxisome proliferation.
| Mitochondria|| |
More than 90% of the oxygen received by aerobic cells is consumed in mitochondria, and only 1%–2% of this oxygen in mammalian mitochondria is used for the production of reactive oxygen intermediates. Thus, oxygen-free radicals and hydroperoxides are being generated continuously as a product of mitochondrial respiratory chain, causing oxidative damage (particularly the hydroxyl radical). The mitochondrial respiratory chain generates O2− anions, which are converted to H2O2 within mitochondria, which will be released outside of the mitochondria. It may cause damage to surrounding structures, especially mitochondrial DNA (mtDNA).
mtDNA is more prone to oxidative damage and mutation, since it lacks protective histones.,, Increased ROS generation in the liver may lead to premature oxidative damage of hepatic mtDNA leading to the development of HCC., The amounts of oxidative stress's damaging effects on mtDNA are several times greater than those of nuclear DNA, since mtDNA's structural properties makes it several times more sensitive to mutations than nuclear DNA. Mitochondrial reduced GSH plays a key role in protecting mtDNA against oxidative damage. Indeed, the oxidative damage to mtDNA is directly related to oxidation of mitochondrial GSH. The respiratory enzymes containing the defective mtDNA-encoded protein subunits may thus increase the ROS production, which in turn aggravates the oxidative damage to mitochondria. O2− radicals produced during mitochondrial respiratory chain's activity also react with NO inside the mitochondria to produce destructive agent “peroxynitrite.” Furthermore, mitochondria are themselves a source of NO, which may increase the formation of O2− radicals and H2O2 by mitochondria. An increase in the production of ROS is responsible for the decline in the activity of mitochondrial membrane proteins thus inhibiting mitochondrial respiratory chain's activity. The activation of stimulatory receptors causing enhanced production of NO and O2− will provide another source of peroxynitrite production. NO inhibits reversibly the activity of respiratory chain at the site of cytochrome C oxidase, on the contrary, peroxynitrite inhibits the activity of respiratory chain irreversibly via inhibition of cytochrome oxidase and complexes I–III.
| Effect of Oxidative Damage on Intracellular Dna, Lipids, and Proteins: Potential Adverse Consequences of Oxidative Stress|| |
Oxidative stress may result in damages of critical cellular macromolecules including DNA, lipids, and proteins. Oxidative DNA injury may participate in ROS-induced carcinogenesis. DNA damage has been observed in a wide range of mammalian cell types exposed to oxidative stress. These damages can occur in different ways including single- and double-stranded DNA breakages, deletions and insertions of single nucleotides, and even chromosomal aberrations. Major molecular mechanisms involved in DNA injuries can occur following the direct reaction of hydroxyl radicals and carbonyl compounds with DNA resulting in the activation of nucleases. Superoxide and H2O2 just can react with DNA in the presence of transitional metal ions which cause hydroxyl radicals formation. The hydroxyl radical may attack to deoxyribose, purines, and pyrimidines, giving rise to numerous products, such as 8-hydroxydeoxyguanosin, thymidine glycol, and 8-hydroxyadenosine. One of the most common forms of DNA injury is the formation of hydroxylated bases in DNA structure, which is considered to be an important event in the chemical carcinogenesis cycle. Formation of such by-products interferes with normal cell growth through genetic mutations and alterations in the ordinary transcription processes of genes. Oxidative DNA injury causes mutations through different pathways, including chemical modification of nucleotide moieties in DNA leading to alterations in their hydrogen bonding, exacerbation of polymerase-specific hot spots, conformational changes in the DNA templates, and the induction of an error-prone DNA polymerase conformation.
Cellular fatty acids may be another target of oxidative stress products and can be readily oxidized by ROS producing lipid peroxyl radicals and lipid hydroperoxides. Lipid peroxyl radicals can subsequently propagate into malondialdehyde (MDA). Moreover, these lipid radicals can diffuse through membranes modifying the structure and the function of the membrane, thus resulting in disruption of cell homeostasis. In addition, lipid peroxides may interact with cellular DNA triggering the formation of DNA-MDA compounds. Lipid damage through lipid peroxidation may result in several possible processes in which the most important is protein oxidation. Proteins are also easily attacked by ROS through lipid peroxidation. Protein-derived radicals can be rapidly transferred to other sites within the protein infrastructure. This can result in further modifications of enzymatic activities. In addition to enzymes, damages to the membrane transport proteins may produce cellular ionic homeostasis and lead to alterations in intercellular calcium and potassium triggering a series of changes in target cells. Alterations in receptor and gap junction proteins may also modify signaling in cells. In some cases, structural changes of proteins may allow the target proteins to be under the further attacks of proteinases.
| Other Targets of Oxidative Stress|| |
Activation of transcription factors is an important signaling pathway for the regulation of gene transcription by ROS. Transcription factors are proteins that can bind to the promoter region of a gene, thus regulating the transcription of genes involved in the development, growth, and aging of cells. Regulation of subcellular localization from cytoplasm to cell nucleus is the first step for transcription factor's activity, which is believed to be involved in this process. Considered to be the target of oxidative stress. Nuclear factor kappa B and activator protein-1 (AP-1) are considered to be amongst the most important targets of oxidative stress. The AP-1 transcription factor controls genes required for cell growth and its activity is increased by compounds with a major role in inducing the cellular proliferation. ROS can cause activation of AP-1 as well as inducing the synthesis of it. Oxidative stress can also increase AP-1 transcription factor's activity, concluding that ROS may play a central role in intracellular signal transduction. High levels of ROS may alter signal pathways through oxidative injury induced in cell membrane, changes in enzymatic activity, and/or the activation of transcription factors. These alterations may create important links between oxidative stress and tumorigenesis. Consequences of ROS production on gene transcription may also inhibit normal cell apoptosis and result in an increase in the number of cells.
| Oxidative Stress Indifferent Stages of Cancer|| |
Induction of cancers through chemicals is a multistage process which can be defined by at least three steps or stages: initiation, promotion, and progression. Initiation stage contains a nonlethal and inheritable mutation in cells by the interaction of a chemical with DNA, conferring an additional growth to target cells. Activation of the carcinogen to an electrophilic DNA-damaging moiety is critical for initiatory stage of DNA injury. ROS compounds are believed to mediate the activation of such carcinogens through hydroperoxide-dependent oxidation that can be mediated by peroxyl radicals. ROS compounds or their derivatives from lipid peroxidation, MDA, can also directly react with DNA to form oxidative DNA adducts. The presence of carcinogen-DNA and oxidative DNA adducts generated through chemical carcinogen's activities suggest an interactive role for ROS in initiation stage. Therefore, ROS can have multiple effects on the initiation stage of carcinogenesis by mediating carcinogen activation, causing DNA injury, and interfering with the repair of the damaged DNA. The second stage (promotion) consists of the selective clonal expansion of the initiatory cell populations through either increased cellular proliferation and/or inhibition of cell death (apoptosis). Promotion stage of tumors will be accompanied by the involvement of selective clonal expansion of the initiatory cell populations through either increased cell division and/or decrease in the occurrence of cell death (apoptosis)., The final stage of tumorigenesis (progression) comprises the development of irreversible cancer growth from the preneoplastic cells of lesions. This results in the formation of the preneoplastic lesions (foci from) as a pathologic consequence of above-mentioned processes. ROS agents are specifically generated in initiatory cell populations such as preneoplastic foci in the liver. Since ROS generation is related to P450 enzyme's activity, oxidative stress may have an important role in the clonal expansion of these initiatory cells. In fact, higher levels of ROS have been found in neoplastic nodules of rat liver in comparison with surrounding normal cells of liver's tissues. Another source of ROS can result from the oxidation of GSH by y-glutamyl transpeptidase in preneoplastic foci. Moreover, extracellular sources of ROS may come from inflammatory cells.
These multiple sources of ROS may contribute to the formation of a persistent oxidative stress environment resulting in pathophysiologic changes and consequently allows for the selective growth of preneoplastic initiatory cells. Tumor progression results in the development of malignant benign lesions. At this point of the progression stage, oxidative stress may directly be effective on the emersion of cancerous lesions characteristics such as uncontrolled growth, genomic instability, chemotherapy resistance, invasion, and metastasis of cancerous cells. Tumor cells continually undergo high and persistent oxidative stress. This persistent oxidative stress does not seem to be effective enough to induce cell death, since tumor cell's sensitivity to oxidative stress is decreased.
| Oxidative Stress and Hepatocarcinogenesis: Causes and Triggers|| |
Viral infection with either hepatitis C virus (HCV) or hepatitis B virus (HBV) is the most common and main cause of LC., One possible mechanism of hepatocarcinogenesis of HCV is the involvement of oxidative stress, triggering genetic mutations as well as chromosomal alterations thus contributing to cancer development.
Viruses cause HCC as a consequence of massive inflammation, fibrosis, and eventual cirrhosis within the liver. numerous genetic and epigenetic alterations occur in liver cells during HCV and HBV infection, considering to be the major factor in the induction of the liver tumors. Viruses induce malignancy inducing changes in cells by altering gene methylation, affecting gene expression and promoting or repressing cellular signal transduction pathways. Thereupon, viruses can prevent apoptosis and promote viral replication and persistency. The presence of HCV may induce the production of ROS itself in human liver and render hepatocytes susceptible to DNA damage, the accumulation of which may lead to malignant transformation. Some mechanisms attributed to the generation of free radicals and increased oxidative stress in HCV-infected individuals include: activation of nicotinamide adenine dinucleotide phosphate hydrogen (NADPH) oxidase within Kupffer cells and polymorphonuclear neutrophil cells during inflammation, iron overload and lipid peroxidation, activation of NADPH oxidase by NS3 protein of HCV, increased production of mitochondrial ROS/RNS by the electron transport chain of core and NS5A proteins of HCV, reduction in GSH output as a consequence of liver injury, decreased antioxidants and related genes expression, enhancement of pro-inflammatory cytokines, increased expression/activity of cyclooxygenase2, amplification in the expression of CYP2E1.,,
Liver is the major site of ethanol metabolism, thus chronic alcohol consumption is associated with progressive liver diseases. In alcohol-related liver disorders, free radicals play a role in the pathogenesis of liver damage. Ethanol consumption increases ROS production, reduces cellular antioxidant levels, and enhances the oxidative stress in many tissues, especially the liver. Acetaldehyde produced through the oxidation of alcohol has got the ability to inhibit the repair of alkylated nucleoproteins, decrease the activity of several enzymes, and causing damages to mitochondria. It also promotes cell death by depleting the levels of reduced GSH through inducing lipid peroxidation, and increasing the toxic effects of free radicals. Finally, acetaldehyde has been shown to enhance collagen synthesis.
Chronic ethanol treatment suppresses mitochondrial function. Alcohol-induced inflammatory and innate immune-mediated responses of Kupffer cells increase ROS-induced injury and fibrinogenesis-inducing factors (e.g., acetaldehyde or lipid peroxidation products).
Aflatoxins are a group of chemicals produced mainly by the fungi Aspergillus flavus and Aspergillus parasiticus. Food contamination by such fungi leads to ingestion of the chemicals. Aflatoxin is a potent hepatotoxic and hepatocarcinogenic agent, that exposure to it can lead to the development of HCC. The mechanism by which aflatoxins cause cancer is through genetic mutations of gene involved in the prevention of cancer called p53. In aflatoxicosis, oxidative stress would be a common mechanism that contributes to the initiation and progression of hepatic damage. It is metabolized in the liver cells and activated by hepatic cytochrome P450 enzyme system to produce a highly reactive intermediate, which subsequently binds to nucleophilic sites of DNA. Moreover, its genotoxic proprieties can induce oxidative stress more than ever.
| Obesity, Diabetes, and Smoking|| |
Several studies have been established a link between smoking, diabetes, and obesity with a state of excess oxidative stress [Figure 1].,,
|Figure 1: Viral infection, obesity, smoking, diabetes, alcohol consumption directly or indirectly affects mitochondrial or peroxisome enzymes to produce reactive species, resulting in the gradual formation of hepatocellular carcinoma|
Click here to view
Obesity has been implicated in the genesis of noncancerous liver diseases, such as nonalcoholic fatty liver disease (NAFLD). However, without proper management, NAFLD may cause severe liver inflammation, termed as nonalcoholic steatohepatitis, which can cause liver fibrosis and cirrhosis with serious complications, including liver failure, and HCC. Epidemiological studies indicated that HCC shows the most strong straight correlation with obesity amongst all other cancers. Fat accumulation and elevated levels of fatty acids correlate with systemic oxidative stress in human beings. Remarkably, obese people display elevated levels of systemic oxidative stress, thus enhancing ROS which occurs in concurrent with lipid accumulation. Thus, adipose tissue represents an important source of ROS and oxidative stress may be a linking factor between obesity and cancer. Epidemiological studies indicated that diabetes mellitus is another risk factor for chronic liver disorders and HCC. Several mechanisms may explain the association between diabetes and primary LC. Patients with insulin-independent form of diabetes (insulin resistant diabetes) showed compensatory hyperinsulinemia, which may stimulate hepatic cell proliferation. Moreover, patients suffering from diabetes may undergo liver alterations, including fatty degeneration and cirrhosis, which favor the process of liver carcinogenesis through the stimulation of cell proliferation. Furthermore, lipid peroxidation is considered as a source of mutagens triggered by ROS. Such condition has been shown to encourage the development of cancer-promoting mutations in diabetic patients.
In addition to the critical role of obesity and diabetes in LCs, many studies have shown a strong association of LCs with smoking as it significantly elevated the risk of HCC. The effect of cigarette smoking on individuals may promote the progression from hepatitis to cirrhosis, or from cirrhosis to HCC. The presence of several compounds in tobacco and the role of liver in the metabolism of these compounds, makes the liver prone to HCC.
| Discussion and Future Perspective|| |
According to recent studies, oxidative stress appears to be an important factor in a number of human diseases including the induction of LC. Several agents seem to induce oxidative stress either directly or indirectly through alterations of cellular antioxidant defense mechanisms. In conclusion, formation of ROS triggered by toxic agents, specifically chemical carcinogens, may be considered as an important mechanism in evaluating LC.
The authors would like to thank Dr. Mehdi Goudarzi for scientific assistance.
Financial support and sponsorship
The study was supported by the Ahvaz Jundishapur University of Medical Sciences for grant 93023.
Conflicts of interest
There are no conflicts of interest.
| References|| |
Ermak G, Davies KJ. Calcium and oxidative stress: From cell signaling to cell death. Mol Immunol 2002;38:713-21.
Schrader M, Fahimi HD. Peroxisomes and oxidative stress. Biochim Biophys Acta 2006;1763:1755-66.
Chance B, Sies H, Boveris A. Hydroperoxide metabolism in mammalian organs. Physiol Rev 1979;59:527-605.
Parke DV, Ioannides C. Role of cytochromes P-450 in mouse liver tumor production. Prog Clin Biol Res 1990;331:215-30.
Halliwell B. Mechanisms involved in the generation of free radicals. Pathol Biol (Paris) 1996;44:6-13.
Trush MA, Kensler TW. An overview of the relationship between oxidative stress and chemical carcinogenesis. Free Radic Biol Med 1991;10:201-9.
Wiseman H, Halliwell B. Damage to DNA by reactive oxygen and nitrogen species: Role in inflammatory disease and progression to cancer. Biochem J 1996;313(Pt 1):17-29.
Drge W. Oxidative Stress and Aging. Hypoxia: Springer; 2003. p. 191-200.
Saran M. To what end does nature produce superoxide? NADPH oxidase as an autocrine modifier of membrane phospholipids generating paracrine lipid messengers. Free Radic Res 2003;37:1045-59.
Patel RP, McAndrew J, Sellak H, White CR, Jo H, Freeman BA, et al.
Biological aspects of reactive nitrogen species. Biochim Biophys Acta 1999;1411:385-400.
Moldovan L, Moldovan NI. Oxygen free radicals and redox biology of organelles. Histochem Cell Biol 2004;122:395-412.
Jezek P, Hlavatá L. Mitochondria in homeostasis of reactive oxygen species in cell, tissues, and organism. Int J Biochem Cell Biol 2005;37:2478-503.
Irshad M, Chaudhuri PS. Oxidant-antioxidant system: Role and significance in human body. Indian J Exp Biol 2002;40:1233-9.
Hu Y, Rosen DG, Zhou Y, Feng L, Yang G, Liu J, et al.
Mitochondrial manganese-superoxide dismutase expression in ovarian cancer: Role in cell proliferation and response to oxidative stress. J Biol Chem 2005;280:39485-92.
Barber DA, Harris SR. Oxygen free radicals and antioxidants: A review. Am Pharm 1994;NS34:26-35.
Vuillaume M. Reduced oxygen species, mutation, induction and cancer initiation. Mutat Res 1987;186:43-72.
Klaunig JE, Xu Y, Isenberg JS, Bachowski S, Kolaja KL, Jiang J, et al.
The role of oxidative stress in chemical carcinogenesis. Environ Health Perspect 1998;106 Suppl 1:289-95.
Ames BN. Dietary carcinogens and anticarcinogens. Oxygen radicals and degenerative diseases. Science 1983;221:1256-64.
Nelson RL. Dietary iron and colorectal cancer risk. Free Radic Biol Med 1992;12:161-8.
Kalantari H, Nazari Z, Keliddar A, Foruozandeh H, Kalantar M. Study of the protective effect of livergol against liver toxicity caused by bromobenzene in mice. Iran J Pharm Sci 2015;10:11-20.
Forouzandeh H, Azemi ME, Rashidi I, Goudarzi M, Kalantari H. Study of the protective effect of Teucrium polium
L. extract on acetaminophen-induced hepatotoxicity in mice. Iran J Pharm Res 2013;12:123-9.
Bosch FX, Ribes J, Díaz M, Cléries R. Primary liver cancer: Worldwide incidence and trends. Gastroenterology 2004;127 5 Suppl 1:S5-16.
Ahmed I, Lobo DN. Malignant tumours of the liver. Surgery (Oxford) 2009;27:30-7.
Khan SA, Davidson BR, Goldin RD, Heaton N, Karani J, Pereira SP, et al.
Guidelines for the diagnosis and treatment of cholangiocarcinoma: An update. Gut 2012;61:1657-69.
De Duve C, Baudhuin P. Peroxisomes (microbodies and related particles). Physiol Rev 1966;46:323-57.
Boveris A, Oshino N, Chance B. The cellular production of hydrogen peroxide. Biochem J 1972;128:617-30.
Stolz DB, Zamora R, Vodovotz Y, Loughran PA, Billiar TR, Kim YM, et al.
Peroxisomal localization of inducible nitric oxide synthase in hepatocytes. Hepatology 2002;36:81-93.
Fahimi HD, Reinicke A, Sujatta M, Yokota S, Ozel M, Hartig F, et al.
The short- and long-term effects of bezafibrate in the rat. Ann N
Y Acad Sci 1982;386:111-35.
Bacon BR, Britton RS. Hepatic injury in chronic iron overload. Role of lipid peroxidation. Chem Biol Interact 1989;70:183-226.
Yokota S, Oda T, Fahimi HD. The role of 15-lipoxygenase in disruption of the peroxisomal membrane and in programmed degradation of peroxisomes in normal rat liver. J Histochem Cytochem 2001;49:613-22.
Reddy JK. Peroxisome proliferators and peroxisome proliferator-activated receptor alpha: Biotic and xenobiotic sensing. Am J Pathol 2004;164:2305-21.
Yeldandi AV, Yeldandi V, Kumar S, Murthy CV, Wang XD, Alvares K, et al.
Molecular evolution of the urate oxidase-encoding gene in hominoid primates: Nonsense mutations. Gene 1991;109:281-4.
Angermüller S, Bruder G, Völkl A, Wesch H, Fahimi HD. Localization of xanthine oxidase in crystalline cores of peroxisomes. A cytochemical and biochemical study. Eur J Cell Biol 1987;45:137-44.
Cancio I, Orbea A, Völkl A, Fahimi HD, Cajaraville MP. Induction of peroxisomal oxidases in mussels: Comparison of effects of lubricant oil and benzo (a) pyrene with two typical peroxisome proliferators on peroxisome structure and function in Mytilus galloprovincialis
. Toxicol Appl Pharmacol 1998;149:64-72.
Thomas T, Thomas TJ. Polyamine metabolism and cancer. J Cell Mol Med 2003;7:113-26.
Zaar K, Köst HP, Schad A, Völkl A, Baumgart E, Fahimi HD. Cellular and subcellular distribution of D-aspartate oxidase in human and rat brain. J Comp Neurol 2002;450:272-82.
Zaar K, Angermüller S, Völkl A, Fahimi HD. Pipecolic acid is oxidized by renal and hepatic peroxisomes. Implications for Zellweger's cerebro-hepato-renal syndrome (CHRS). Exp Cell Res 1986;164:267-71.
Chikayama M, Ohsumi M, Yokota S. Enzyme cytochemical localization of sarcosine oxidase activity in the liver and kidney of several mammals. Histochem Cell Biol 2000;113:489-95.
Recalcati S, Menotti E, Kühn LC. Peroxisomal targeting of mammalian hydroxyacid oxidase 1 requires the C-terminal tripeptide SKI. J Cell Sci 2001;114(Pt 9):1625-9.
Recalcati S, Tacchini L, Alberghini A, Conte D, Cairo G. Oxidative stress-mediated down-regulation of rat hydroxyacid oxidase 1, a liver-specific peroxisomal enzyme. Hepatology 2003;38:1159-66.
Loughran PA, Stolz DB, Vodovotz Y, Watkins SC, Simmons RL, Billiar TR. Monomeric inducible nitric oxide synthase localizes to peroxisomes in hepatocytes. Proc Natl Acad Sci U S A 2005;102:13837-42.
Oshino N, Chance B, Sies H, Bücher T. The role of H2
generation in perfused rat liver and the reaction of catalase compound I and hydrogen donors. Arch Biochem Biophys 1973;154:117-31.
Siraki AG, Pourahmad J, Chan TS, Khan S, O'Brien PJ. Endogenous and endobiotic induced reactive oxygen species formation by isolated hepatocytes. Free Radic Biol Med 2002;32:2-10.
Litwin JA, Beier K, Vlkl A, Hofmann WJ, Fahimi HD. Immunocytochemical investigation of catalase and peroxisomal lipid-oxidation enzymes in human hepatocellular tumors and liver cirrhosis. Virchows Arch 1999;435:486-95.
Winterbourn CC. Toxicity of iron and hydrogen peroxide: The Fenton reaction. Toxicol Lett 1995;82-83:969-74.
Zhou Y, Hileman EO, Plunkett W, Keating MJ, Huang P. Free radical stress in chronic lymphocytic leukemia cells and its role in cellular sensitivity to ROS-generating anticancer agents. Blood 2003;101:4098-104.
Yoshioka T, Homma T, Meyrick B, Takeda M, Moore-Jarrett T, Kon V, et al.
Oxidants induce transcriptional activation of manganese superoxide dismutase in glomerular cells. Kidney Int 1994;46:405-13.
Church SL, Grant JW, Ridnour LA, Oberley LW, Swanson PE, Meltzer PS, et al.
Increased manganese superoxide dismutase expression suppresses the malignant phenotype of human melanoma cells. Proc Natl Acad Sci U S A 1993;90:3113-7.
Schadendorf D, Zuberbier T, Diehl S, Schadendorf C, Czarnetzki BM. Serum manganese superoxide dismutase is a new tumour marker for malignant melanoma. Melanoma Res 1995;5:351-3.
Biswal BK, Morisseau C, Garen G, Cherney MM, Garen C, Niu C, et al.
The molecular structure of epoxide hydrolase B from Mycobacterium tuberculosis
and its complex with a urea-based inhibitor. J Mol Biol 2008;381:897-912.
Yamashita H, Avraham S, Jiang S, London R, Van Veldhoven PP, Subramani S, et al.
Characterization of human and murine PMP20 peroxisomal proteins that exhibit antioxidant activity in vitro
. J Biol Chem 1999;274:29897-904.
Reddy JK, Lalwai ND. Carcinogenesis by hepatic peroxisome proliferators: Evaluation of the risk of hypolipidemic drugs and industrial plasticizers to humans. Crit Rev Toxicol 1983;12:1-58.
Beier K, Fahimi HD. Environmental pollution by common chemicals and peroxisome proliferation: Efficient detection by cytochemistry and automatic image analysis. Prog Histochem Cytochem 1991;23:150-63.
Issemann I, Green S. Activation of a member of the steroid hormone receptor superfamily by peroxisome proliferators. Nature 1990;347:645-50.
Lake BG. Role of oxidative stress and enhanced cell replication in the hepatocarcinogenicity of peroxisome proliferators. In: Gibson G, Lake BG ed. Peroxisomes: Biology and importance in toxicology and medicine. Taylor and Francis, London. 1993. p. 595-618.
Roberts RA. Non-genotoxic hepatocarcinogenesis: Suppression of apoptosis by peroxisome proliferators. Ann N
Y Acad Sci 1996;804:588-611.
Rose ML, Rusyn I, Bojes HK, Belyea J, Cattley RC, Thurman RG. Role of Kupffer cells and oxidants in signaling peroxisome proliferator-induced hepatocyte proliferation. Mutat Res 2000;448:179-92.
Klaunig JE, Babich MA, Baetcke KP, Cook JC, Corton JC, David RM, et al.
PPARalpha agonist-induced rodent tumors: Modes of action and human relevance. Crit Rev Toxicol 2003;33:655-780.
Sastre J, Pallardó FV, Viña J. Mitochondrial oxidative stress plays a key role in aging and apoptosis. IUBMB Life 2000;49:427-35.
Forman HJ, Azzi A. On the virtual existence of superoxide anions in mitochondria: Thoughts regarding its role in pathophysiology. FASEB J 1997;11:374-5.
Flier JS, Underhill LH, Johns DR. Mitochondrial DNA and disease. N Engl J Med 1995;333:638-44.
Lee CM, Weindruch R, Aiken JM. Age-associated alterations of the mitochondrial genome. Free Radic Biol Med 1997;22:1259-69.
Sastre J, Pallardó FV, Plá R, Pellín A, Juan G, O'Connor JE, et al.
Aging of the liver: Age-associated mitochondrial damage in intact hepatocytes. Hepatology 1996;24:1199-205.
Adler V, Yin Z, Tew KD, Ronai Z. Role of redox potential and reactive oxygen species in stress signaling. Oncogene 1999;18:6104-11.
Yan LJ, Levine RL, Sohal RS. Oxidative damage during aging targets mitochondrial aconitase. Proc Natl Acad Sci U S A 1997;94:11168-72.
Richter C, Park JW, Ames BN. Normal oxidative damage to mitochondrial and nuclear DNA is extensive. Proc Natl Acad Sci U S A 1988;85:6465-7.
de la Asuncion JG, Millan A, Pla R, Bruseghini L, Esteras A, Pallardo FV, et al.
Mitochondrial glutathione oxidation correlates with age-associated oxidative damage to mitochondrial DNA. FASEB J 1996;10:333-8.
Wei YH. Oxidative stress and mitochondrial DNA mutations in human aging. Exp Biol Med 1998;217:53-63.
Keller JN, Kindy MS, Holtsberg FW, St. Clair DK, Yen HC, Germeyer A, et al.
Mitochondrial manganese superoxide dismutase prevents neural apoptosis and reduces ischemic brain injury: Suppression of peroxynitrite production, lipid peroxidation, and mitochondrial dysfunction. J Neurosci 1998;18:687-97.
Corbisier P, Raes M, Michiels C, Pigeolet E, Houbion A, Delaive E, et al.
Respiratory activity of isolated rat liver mitochondria following in vitro
exposure to oxygen species: A threshold study. Mech Ageing Dev 1990;51:249-63.
Takeyama N, Matsuo N, Tanaka T. Oxidative damage to mitochondria is mediated by the Ca2+-dependent inner-membrane permeability transition. Biochem J 1993;294(Pt 3):719-25.
Bolaños JP, Almeida A, Stewart V, Peuchen S, Land JM, Clark JB, et al.
Nitric oxide-mediated mitochondrial damage in the brain: Mechanisms and implications for neurodegenerative diseases. J Neurochem 1997;68:2227-40.
Brown GC. Nitric oxide regulates mitochondrial respiration and cell functions by inhibiting cytochrome oxidase. FEBS Lett 1995;369:136-9.
Sharpe MA, Cooper CE. Interaction of peroxynitrite with mitochondrial cytochrome oxidase. Catalytic production of nitric oxide and irreversible inhibition of enzyme activity. J Biol Chem 1998;273:30961-72.
Breimer LH. Molecular mechanisms of oxygen radical carcinogenesis and mutagenesis: The role of DNA base damage. Mol Carcinog 1990;3:188-97.
Halliwell B, Aruoma OI. DNA damage by oxygen-derived species. Its mechanism and measurement in mammalian systems. FEBS Lett 1991;281:9-19.
Chaudhary AK, Nokubo M, Marnett LJ, Blair IA. Analysis of the malondialdehyde-2'-deoxyguanosine adduct in rat liver DNA by gas chromatography/electron capture negative chemical ionization mass spectrometry. Biol Mass Spectrom 1994;23:457-64.
Feig DI, Reid TM, Loeb LA. Reactive oxygen species in tumorigenesis. Cancer Res 1994;54 7 Suppl: 1890s-4s.
Rice-Evans C, Burdon R. Free radical-lipid interactions and their pathological consequences. Prog Lipid Res 1993;32:71-110.
Bellomo G, Mirabelli F, Richelmi P, Orrenius S. Critical role of sulfhydryl group(s) in ATP-dependent Ca2+ sequestration by the plasma membrane fraction from rat liver. FEBS Lett 1983;163:136-9.
Kerr LD, Inoue J, Verma IM. Signal transduction: The nuclear target. Curr Opin Cell Biol 1992;4:496-501.
Davies KJ. Intracellular proteolytic systems may function as secondary antioxidant defenses: An hypothesis. J Free Radic Biol Med 1986;2:155-73.
Storz G, Polla B. Transcriptional regulators of oxidative stress-inducible genes in prokaryotes and eukaryotes. EXS 1996;77:239-54.
Vellanoweth RL, Suprakar PC, Roy AK. Transcription factors in development, growth, and aging. Lab Invest 1994;70:784-99.
Whiteside ST, Goodbourn S. Signal transduction and nuclear targeting: Regulation of transcription factor activity by subcellular localisation. J Cell Sci 1993;104(Pt 4):949-55.
Schenk H, Klein M, Erdbrügger W, Dröge W, Schulze-Osthoff K. Distinct effects of thioredoxin and antioxidants on the activation of transcription factors NF-kappa B and AP-1. Proc Natl Acad Sci U S A 1994;91:1672-6.
Ames BN, Gold LS. Animal cancer tests and cancer prevention. J Natl Cancer Inst Monogr 1992;12:125-32.
Schulte-Hermann R, Timmermann-Trosiener I, Barthel G, Bursch W. DNA synthesis, apoptosis, and phenotypic expression as determinants of growth of altered foci in rat liver during phenobarbital promotion. Cancer Res 1990;50:5127-35.
Guyton KZ, Kensler TW. Oxidative mechanisms in carcinogenesis. Br Med Bull 1993;49:523-44.
Scholz W, Schütze K, Kunz W, Schwarz M. Phenobarbital enhances the formation of reactive oxygen in neoplastic rat liver nodules. Cancer Res 1990;50:7015-22.
Cerutti P, Amstad P. Inflammation and oxidative stress in carcinogenesis. Eicosanoids and Other Bioactive Lipids in Cancer, Inflammation and Radiation Injury. US: Springer; 1993. p. 387-90.
Toyokuni S, Okamoto K, Yodoi J, Hiai H. Persistent oxidative stress in cancer. FEBS Lett 1995;358:1-3.
Palozza P, Agostara G, Piccioni E, Bartoli GM. Different role of lipid peroxidation in oxidative stress-induced lethal injury in normal and tumor thymocytes. Arch Biochem Biophys 1994;312:88-94.
Arzumanyan A, Reis HM, Feitelson MA. Pathogenic mechanisms in HBV- and HCV-associated hepatocellular carcinoma. Nat Rev Cancer 2013;13:123-35.
Rosen HR. Clinical practice. Chronic hepatitis C infection. N Engl J Med 2011;364:2429-38.
Moriya K, Nakagawa K, Santa T, Shintani Y, Fujie H, Miyoshi H, et al.
Oxidative stress in the absence of inflammation in a mouse model for hepatitis C virus-associated hepatocarcinogenesis. Cancer Res 2001;61:4365-70.
Jeong SW, Jang JY, Chung RT. Hepatitis C virus and hepatocarcinogenesis. Clin Mol Hepatol 2012;18:347-56.
Choi J, Ou JH. Mechanisms of liver injury. III. Oxidative stress in the pathogenesis of hepatitis C virus. Am J Physiol Gastrointest Liver Physiol 2006;290:G847-51.
Machida K, Cheng KT, Sung VM, Lee KJ, Levine AM, Lai MM. Hepatitis C virus infection activates the immunologic (type II) isoform of nitric oxide synthase and thereby enhances DNA damage and mutations of cellular genes. J Virol 2004;78:8835-43.
Thorén F, Romero A, Lindh M, Dahlgren C, Hellstrand K. A hepatitis C virus-encoded, nonstructural protein (NS3) triggers dysfunction and apoptosis in lymphocytes: Role of NADPH oxidase-derived oxygen radicals. J Leukoc Biol 2004;76:1180-6.
Shepard BD, Tuma DJ, Tuma PL. Chronic ethanol consumption induces global hepatic protein hyperacetylation. Alcohol Clin Exp Res 2010;34:280-91.
Wu D, Cederbaum AI. Oxidative stress and alcoholic liver disease. Semin Liver Dis 2009;29:141-54.
Lieber CS. Alcoholic fatty liver: Its pathogenesis and mechanism of progression to inflammation and fibrosis. Alcohol 2004;34:9-19.
Kato J, Sato Y, Inui N, Nakano Y, Takimoto R, Takada K, et al.
Ethanol induces transforming growth factor-alpha expression in hepatocytes, leading to stimulation of collagen synthesis by hepatic stellate cells. Alcohol Clin Exp Res 2003;27 8 Suppl: 58S-63S.
Cubero FJ, Urtasun R, Nieto N. Alcohol and liver fibrosis. Semin Liver Dis 2009;29:211-21.
Brahmi D, Bouaziz C, Ayed Y, Ben Mansour H, Zourgui L, Bacha H. Chemopreventive effect of cactus Opuntia ficus indica
on oxidative stress and genotoxicity of aflatoxin B1. Nutr Metab (Lond) 2011;8:73.
Kensler TW, Roebuck BD, Wogan GN, Groopman JD. Aflatoxin: A 50-year odyssey of mechanistic and translational toxicology. Toxicol Sci 2011;120 Suppl 1:S28-48.
Sharma RA, Farmer PB. Biological relevance of adduct detection to the chemoprevention of cancer. Clin Cancer Res 2004;10:4901-12.
Frei B, Forte TM, Ames BN, Cross CE. Gas phase oxidants of cigarette smoke induce lipid peroxidation and changes in lipoprotein properties in human blood plasma. Protective effects of ascorbic acid. Biochem J 1991;277(Pt 1):133-8.
Keaney JF Jr., Larson MG, Vasan RS, Wilson PW, Lipinska I, Corey D, et al.
Obesity and systemic oxidative stress: Clinical correlates of oxidative stress in the Framingham Study. Arterioscler Thromb Vasc Biol 2003;23:434-9.
Chuang SC, La Vecchia C, Boffetta P. Liver cancer: Descriptive epidemiology and risk factors other than HBV and HCV infection. Cancer Lett 2009;286:9-14.
Sun B, Karin M. Obesity, inflammation, and liver cancer. J Hepatol 2012;56:704-13.
Larsson SC, Wolk A. Overweight, obesity and risk of liver cancer: A meta-analysis of cohort studies. Br J Cancer 2007;97:1005-8.
Furukawa S, Fujita T, Shimabukuro M, Iwaki M, Yamada Y, Nakajima Y, et al.
Increased oxidative stress in obesity and its impact on metabolic syndrome. J Clin Invest 2004;114:1752-61.
Matsuda M, Shimomura I. Increased oxidative stress in obesity: Implications for metabolic syndrome, diabetes, hypertension, dyslipidemia, atherosclerosis, and cancer. Obes Res Clin Pract 2013;7:e330-41.
El-Serag HB, Tran T, Everhart JE. Diabetes increases the risk of chronic liver disease and hepatocellular carcinoma. Gastroenterology 2004;126:460-8.
Adami HO, Chow WH, Nyrén O, Berne C, Linet MS, Ekbom A, et al.
Excess risk of primary liver cancer in patients with diabetes mellitus. J Natl Cancer Inst 1996;88:1472-7.
La Vecchia C, Negri E, Decarli A, Franceschi S. Diabetes mellitus and the risk of primary liver cancer. Int J Cancer 1997;73:204-7.
Caldwell SH, Crespo DM, Kang HS, Al-Osaimi AM. Obesity and hepatocellular carcinoma. Gastroenterology 2004;127 5 Suppl 1:S97-103.
Fujita Y, Shibata A, Ogimoto I, Kurozawa Y, Nose T, Yoshimura T, et al.
The effect of interaction between hepatitis C virus and cigarette smoking on the risk of hepatocellular carcinoma. Br J Cancer 2006;94:737-9.
Jee SH, Ohrr H, Sull JW, Samet JM. Cigarette smoking, alcohol drinking, hepatitis B, and risk for hepatocellular carcinoma in Korea. J Natl Cancer Inst 2004;96:1851-6.
[Table 1], [Table 2]