Close
  Indian J Med Microbiol
 

Figure 1: Representative images of methylation-specific polymerase chain reaction analysis for (a) secreted frizzled-related protein 1 methylation and (b) Dickkopf 3 methylation in breast carcinoma tissues. Bisulfite modified DNA was amplified using methylation-specific polymerase chain reaction primers specific to a CpG rich region of each gene promoter. Polymerase chain reaction-amplified products were resolved by 2% of agarose gel electrophoresis. DNA bands in lanes labeled with U indicate polymerase chain reaction products amplified with primers recognizing unmethylated promoter sequences. DNA bands in lanes labeled with M represent amplified products with methylation-specific primers

Figure 1: Representative images of methylation-specific polymerase chain reaction analysis for (a) secreted frizzled-related protein 1 methylation and (b) Dickkopf 3 methylation in breast carcinoma tissues. Bisulfite modified DNA was amplified using methylation-specific polymerase chain reaction primers specific to a CpG rich region of each gene promoter. Polymerase chain reaction-amplified products were resolved by 2% of agarose gel electrophoresis. DNA bands in lanes labeled with U indicate polymerase chain reaction products amplified with primers recognizing unmethylated promoter sequences. DNA bands in lanes labeled with M represent amplified products with methylation-specific primers