Submit Your Article CMED MEACR meeting
An official publication of the Middle-Eastern Association for Cancer Research
Clinical Cancer Investigation Journal
ISSN Print: 2278-1668, Online: 2278-0513
ARTICLE
Year: 2019   |   Volume: 8   |   Issue: 3   |   Page: 90-95     View issue

Correlation between ki-67 labeling index and mitotic index in oral squamous cell carcinoma


, , , ,
Abstract

Background: Oral squamous cell carcinoma (OSCC) is the most prevalent type of oral cancer. Counting of mitotic figures as well as the detection of Ki-67 expression by immunohistochemistry have been used to detect cell proliferation in malignant tumors. We correlated Ki-67 expression with mitotic activity and observed any notable relationship between them, to ascertain the role of Ki-67 protein as a tumor proliferative marker in OSCC. Subjects and Methods: A cross-sectional study was undertaken at a tertiary care hospital in Western Maharashtra over a period of 2 years. Fifty paraffin blocks of patients diagnosed as OSCC were included in our study, who were classified into well-differentiated squamous cell carcinoma (n = 24), moderately-differentiated squamous cell carcinoma (n = 23), and poorly differentiated squamous cell carcinoma (n = 3). Two slides per case were stained with H and E and Ki-67 marker, then observed under light microscopy to obtain Mitotic Index (MI) and Ki-67 Labeling Index (LI), respectively. Results were analyzed using mean, standard deviation, Chi-square test, and unpaired t-test. Results: Hyperplastic epithelium revealed higher Ki-67 LI as compared to normal epithelium. There was a statistically significant (P = 0.045) increase in Ki-67 LI in the proliferating margin with grade of OSCC. Positive linear correlation was noted between Ki-67 and MI in tumor proper (r = 0.186) and proliferating margin (r = 0.337) and was statistically significant in the latter (P = 0.017). Conclusions: Ki-67 LI can reliably detect the proliferative potential of cells at the invasive margin of a tumor. MI, on the other hand, can detect the cell proliferation rate of the tumor that is in correlation with the histopathological grade of OSCC.

Cite this article
Vancouver
Buch A, Haldar N, Kheur S, Chandanwale S, Kumar H. Correlation between ki-67 labeling index and mitotic index in oral squamous cell carcinoma. Clin Cancer Investig J. 2019;8(3):90-5. https://doi.org/10.4103/ccij.ccij_23_19
APA
Buch, A., Haldar, N., Kheur, S., Chandanwale, S., & Kumar, H. (2019). Correlation between ki-67 labeling index and mitotic index in oral squamous cell carcinoma. Clinical Cancer Investigation Journal, 8(3), 90-95. https://doi.org/10.4103/ccij.ccij_23_19

© Clinical Cancer Investigation Journal
Online since 01 December, 2011
Creative Commons License 
ISSN Print: 2278-1668, Online: 2278-0513