Clinical Cancer Investigation Journal

ORIGINAL ARTICLE
Year
: 2019  |  Volume : 8  |  Issue : 3  |  Page : 90--95

Correlation between ki-67 labeling index and mitotic index in oral squamous cell carcinoma


Archana Buch, Niladri Haldar, Supriya Kheur, Shirish Chandanwale, Harsh Kumar 
 Dr. D. Y. Patil Medical College, Hospital and Research Centre, Dr. D. Y. Patil Vidyapeeth, Pune, Maharashtra, India

Correspondence Address:
Niladri Haldar
C/o Dr. N. R. Haldar, 11 JK Dutta by Lane, Ramakrishna Road, Ashrampara, Siliguri - 734 001, West Bengal
India

Background: Oral squamous cell carcinoma (OSCC) is the most prevalent type of oral cancer. Counting of mitotic figures as well as the detection of Ki-67 expression by immunohistochemistry have been used to detect cell proliferation in malignant tumors. We correlated Ki-67 expression with mitotic activity and observed any notable relationship between them, to ascertain the role of Ki-67 protein as a tumor proliferative marker in OSCC. Subjects and Methods: A cross-sectional study was undertaken at a tertiary care hospital in Western Maharashtra over a period of 2 years. Fifty paraffin blocks of patients diagnosed as OSCC were included in our study, who were classified into well-differentiated squamous cell carcinoma (n = 24), moderately-differentiated squamous cell carcinoma (n = 23), and poorly differentiated squamous cell carcinoma (n = 3). Two slides per case were stained with H and E and Ki-67 marker, then observed under light microscopy to obtain Mitotic Index (MI) and Ki-67 Labeling Index (LI), respectively. Results were analyzed using mean, standard deviation, Chi-square test, and unpaired t-test. Results: Hyperplastic epithelium revealed higher Ki-67 LI as compared to normal epithelium. There was a statistically significant (P = 0.045) increase in Ki-67 LI in the proliferating margin with grade of OSCC. Positive linear correlation was noted between Ki-67 and MI in tumor proper (r = 0.186) and proliferating margin (r = 0.337) and was statistically significant in the latter (P = 0.017). Conclusions: Ki-67 LI can reliably detect the proliferative potential of cells at the invasive margin of a tumor. MI, on the other hand, can detect the cell proliferation rate of the tumor that is in correlation with the histopathological grade of OSCC.


How to cite this article:
Buch A, Haldar N, Kheur S, Chandanwale S, Kumar H. Correlation between ki-67 labeling index and mitotic index in oral squamous cell carcinoma.Clin Cancer Investig J 2019;8:90-95


How to cite this URL:
Buch A, Haldar N, Kheur S, Chandanwale S, Kumar H. Correlation between ki-67 labeling index and mitotic index in oral squamous cell carcinoma. Clin Cancer Investig J [serial online] 2019 [cited 2019 Oct 20 ];8:90-95
Available from: http://www.ccij-online.org/article.asp?issn=2278-0513;year=2019;volume=8;issue=3;spage=90;epage=95;aulast=Buch;type=0