Clinical Cancer Investigation Journal

ORIGINAL ARTICLE
Year
: 2015  |  Volume : 4  |  Issue : 5  |  Page : 603--609

Children with acute lymphoblastic leukemia show high numbers of CD4+ and CD8+ T-cells which are reduced by conventional chemotherapy


Mohamed Labib Salem1, Mohamed Ramadan El-Shanshory2, Nabila Ibrahim El-Desouki3, Said Hammad Abdou4, Mohamed Attia Attia4, Abdel-Aziz Awad Zidan5, Shymaa Sobhy Mourad1 
1 Center of Excellence in Cancer Research, Tanta University Educational Hospital; Immunology and Biotechnology Division, Zoology Department, Faculty of Science, Tanta University, Tanta, Egypt
2 Center of Excellence in Cancer Research, Tanta University Educational Hospital; Hematology/Oncology Unit, Pediatric Department, Faculty of Medicine, Tanta University, Tanta, Egypt
3 Histology and Histochemistry Division, Zoology Department, Faculty of Science, Tanta University,Tanta, Egypt
4 Center of Excellence in Cancer Research, Tanta University Educational Hospital; Clinical Pathology, Faculty of Medicine, Medical Campus, Tanta University, Tanta, Egypt
5 Center of Excellence in Cancer Research, Tanta University Educational Hospital, Tanta, Egypt

Correspondence Address:
Mohamed Labib Salem
Center of Excellence in Cancer Research, Tanta University Educational Hospital, Tanta, Egypt.
Egypt

Background: Acute lymphoblastic leukemia (ALL) is considered as one of the most common cancer in pediatric malignancies. Among ALL, B-cell Acute Lymphoblastic Leukemia (B-ALL) represents 80% to 85% of the childhood ALL. Problem: Although anti B-ALL chemotherapy kill B-ALL, it associates with alteration in the numbers of CD4+ and CD8+ T-cells, and thus impacts the overall immunity. Aim: To evaluate the impact of anti B-ALL on the numbers of CD4+ and CD8+ T-cells in correlation to the numbers of CD10+ B cells in B-ALL pediatric patients. Materials and Methods: Peripheral blood samples were drawn from previously diagnosed B-ALL before (n = 10 cases) and after (n = 10 cases) chemotherapy as well as from healthy controls (n = 10 cases). The numbers of CD4+, CD8+ T-cells and CD10+ B cells were measured in these samples by flow cytometry. Results: As expected, the numbers of CD10+ B-cells were increased in B-ALL patients before chemotherapy which were associated with increases in the numbers of CD4+ and CD8+ T-cells. Chemotherapy of B-ALL patients, during the induction phase, induced dramatic decreases in the numbers of CD10+ B cells, which were associated with decreases in the numbers of CD4+ and CD8+ T-cells. Tin spite of this alteration, the ratio of CD4/CD8 in B-ALL patients were remained similar before and after chemotherapy as compared to those in healthy controls. Conclusion: Anti B-ALL chemotherapy induces alterations in the frequencies of T-cell subsets. Given the importance of these cells in anti-tumor immunity, our data may lead to further studies to investigate the different subsets of these cells, in particular regulatory T-cells.


How to cite this article:
Salem ML, El-Shanshory MR, El-Desouki NI, Abdou SH, Attia MA, Zidan AAA, Mourad SS. Children with acute lymphoblastic leukemia show high numbers of CD4+ and CD8+ T-cells which are reduced by conventional chemotherapy.Clin Cancer Investig J 2015;4:603-609


How to cite this URL:
Salem ML, El-Shanshory MR, El-Desouki NI, Abdou SH, Attia MA, Zidan AAA, Mourad SS. Children with acute lymphoblastic leukemia show high numbers of CD4+ and CD8+ T-cells which are reduced by conventional chemotherapy. Clin Cancer Investig J [serial online] 2015 [cited 2020 Jun 3 ];4:603-609
Available from: http://www.ccij-online.org/article.asp?issn=2278-0513;year=2015;volume=4;issue=5;spage=603;epage=609;aulast=Salem;type=0