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ORIGINAL ARTICLE
Year : 2020  |  Volume : 9  |  Issue : 4  |  Page : 110-113

Comparison and frequency of cell cultured in 48-h and 72-h mitotically treated with phytohemagglutinin-M: Both promise for normal and cancer cells


1 Social Determinants of Health Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2 Department of Biology, Zanjan Branch, Islamic Azad University, Zanjan, Iran
3 Department of Mycobacteriology and Pulmonary Research, Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran
4 Proteomics Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
5 Cancer Research Center, Department of Pathology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
6 Department of Cellular-Molecular Biology, Faculty of Biological Sciences and Technologies, Shahid Beheshti University, Tehran, Iran
7 Department of Medical Bactriology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
8 Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
9 Department of Medical Genetics, School of Medicine, International Campus, Tehran University of Medical Sciences, Tehran, Iran
10 Molecular Medicine Department, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia
11 Department of Genetics, Faculty of advanced Sciences and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
12 Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Correspondence Address:
Abolfazl Movafagh
Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran
Iran
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/ccij.ccij_6_20

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The aim of the present study is hence to comprise of mitotic index outcome in the individuals of normal volunteers involved in two different period of culture with effect of Phytohemagglutinin-M. Comparison of healthy human donors lymphocytes were cultured for 48-h and 72-h and using conventional karyotype technique with minor modification. Mitotic index and cell proliferation frequency were analyzed in the individuals of normal blood donors after preparing of colchicine to cultured metaphases 2 h before cell fixation. The metaphase indices at 48-h and 72-h culture period resulted a perfect yield product. Lymphocytes populations in 72-h laboratory work were more than 48-h cultures (2.73% at 72 h vs. 1.48% at 48 h, P ≤ 0.05). The present research indicates significant comparisons for laboratories and individuals to be set up. With effect of these results, it may help in time-consuming and short time period for patient, as well as fresh suggestion for health-care management decision. Reagent, manual, budget, time for diagnosis, and health-care fulfillment may resolve of this proposal.


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