|Year : 2019 | Volume
| Issue : 1 | Page : 28-32
Cytogenetic and molecular assessment of childhood acute lymphoblastic leukemia patients from 2014 to 2017 in Ahvaz
Arash Alqasi, Yousef Tavakolifar, Hadi Rezaeeyan, Najmaldin Saki, Soheila Bagherpour, Marziyeh Abbasi Nasab
Thalassemia and Hemoglobinopathy Research Center, Research Institute of Health, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
|Date of Web Publication||4-Apr-2019|
Dr. Arash Alqasi
Thalassemia and Hemoglobinopathy Research Center, Research Institute of Health, Ahvaz Jundishapur University of Medical Sciences, Ahvaz
Source of Support: None, Conflict of Interest: None
Background: Acute lymphoblastic leukemia (ALL) is the most common hematological malignancy in children that is caused by gene mutations and chromosomal rearrangements in lymphoid cells. Aim: In this study, for the first time, the prevalence of cytogenetic and molecular genetic abnormalities was discussed in children with ALL from 2014 to 2017 in Ahvaz. Materials and Methods: A total of 72 children were diagnosed as ALL patients by morphology, clinical examinations, and flow cytometry assays. Cytogenetic and molecular genetic analysis was done on bone marrow (BM) samples by BM culture and reverse transcription-polymerase chain reaction technique, respectively. Descriptive data analysis was done using SPSS software. Chi-square and independent samples t-test was used to assess the correlation between variables. Results: Sixty-five cases (90.3%) were preB lineage and 7 cases (9.7%) were T-lineage out of 72 ALL patients, t(9,22) BCR-ABL (p190) is the most frequent cytogenetic and molecular genetic abnormality in preB ALL (7%) and T-ALL patients (28.6%), respectively. t(4,3) inv (16) and t(2,8) were introduced as novel cytogenetic abnormalities in preB ALL cells. No significant correlation was found between gender, molecular genetic abnormalities, and white blood cell count in patients. Conclusion: For the first time in this study, the highest percentage of cytogenetic and molecular genetic abnormalities was related to t(9,22) BCR-ABL in both ALL subtypes in children. The evaluation of cytogenetic and molecular genetic abnormalities in children with ALL is essential in estimating the prognosis in both preB and T-ALL subtypes, which will be a great contribution to achieve a better diagnosis and develop appropriate therapeutic approaches.
Keywords: Acute lymphoblastic leukemia, childhood, cytogenetic, molecular
|How to cite this article:|
Alqasi A, Tavakolifar Y, Rezaeeyan H, Saki N, Bagherpour S, Nasab MA. Cytogenetic and molecular assessment of childhood acute lymphoblastic leukemia patients from 2014 to 2017 in Ahvaz. Clin Cancer Investig J 2019;8:28-32
|How to cite this URL:|
Alqasi A, Tavakolifar Y, Rezaeeyan H, Saki N, Bagherpour S, Nasab MA. Cytogenetic and molecular assessment of childhood acute lymphoblastic leukemia patients from 2014 to 2017 in Ahvaz. Clin Cancer Investig J [serial online] 2019 [cited 2019 Apr 24];8:28-32. Available from: http://www.ccij-online.org/text.asp?2019/8/1/28/255440
| Introduction|| |
Acute lymphoblastic leukemia (ALL) is a hematological malignancy with high prevalence among children and is characterized by genetic changes such as mutations and chromosomal translocations. These malignancies are responsible for approximately 80% and 20% of cases of cancers in children and adults, respectively. The ALL outbreak in men and women is 1.7/100,000 per year. The main cause of the disease has not been determined yet, but it has been shown that various factors, including environmental factors, viral infections, and genetic changes, and some syndromes such as Down, Klinefelter, and Bloom have been shown to occur.,,, However, chromosomal translocations and related molecular variations have been shown to play a major role in pathogenesis and therapeutic response in patients. Most of these changes occur in genes that play essential roles in lymphoid development, cell cycle, or as tumor suppressors or oncogene. Genetic changes and clinical symptoms can be useful in the classification of ALL to subtypes as well as diagnostic and prognostic factors for patients monitoring.,, Identifying chromosomal translocations and related molecular changes not only identify leukemia cells pathogenicity, but also optimize therapeutic approach to increase patient survival. Therapeutic approaches are usually based on the prognostic characteristics of chromosomal translocations and their classification based on high-, moderate-, and low-risk groups. For example, recent studies have shown t(1; 19) (q23; p13) associated with the formation of TCF3-PBX1 fusion was associated with a poor prognosis in childhood B-ALL, although new antimetabolic drugs used in chemotherapy increased patients survival due to appropriate therapeutic response., Several chromosomal translocations decrease therapeutic responses and increase mortality in patients. Studies showed t(9; 22) (q34, q11.2) translocation leads to BCR and ABL1 genes fusion, and ultimately leads to the formation of the Philadelphia chromosome (Ph). Ph +-ALL patients have a poor prognosis and are resistant to tyrosine kinase inhibitors. Therefore, the detection of t(9; 22) (q34, q11.2) translocation in ALL patients can increase the survival of patients before developing the disease by employing optimal therapeutic strategies., Genetic changes associated with blood cell counting can be effective in determining the survival of the patients, and in applying therapeutic strategies that prevent the disease progression. Therefore, in this study, we monitored the incidence of cytogenetic and molecular genetic abnormalities in children with ALL, as well as the relationship of genetic abnormalities with laboratory parameters such as white blood cell (WBC) from 2014 to 2017 in Ahvaz.
| Materials and Methods|| |
Study group: Patients and samples
ALL was diagnosed through bone marrow (BM) aspirate containing at least 30% blast cells based on the French–American–British classification during 2014 and 2017 in Ahvaz. After morphologic and clinical examinations, as well as flow cytometry assays on 72 ALL patients, they were enrolled in this study. ALL patients included 46 males (63.9%) and 26 females (36.1%) (5–14-year-old; median age: 5.38 years). Sixty-five cases (90.3%) were preB lineage and 7 (9.7%) were T-lineage ALL. Patients were treated based on ALL protocol in Shifa Hospital and received induction therapy with a combination of drugs, including vincristine, prednisone, cyclophosphamide, doxorubicin, and L-asparaginase. In addition, BM samples were obtained on the 7th day of treatment. A volume of 5 mL of BM sample was collected from each patient in falcon tubes containing heparin and ethylenediaminetetraacetic acid anticoagulants for performing cytogenetic and molecular genetic analyses, respectively. All BM samples were taken within 4 months with written informed consent from the patients. This study was approved by the Local Ethics Committee (IR. AJUMS. REC.1395.485) and was conducted within 8 months.
Cytogenetic and molecular genetic analyses
Cytogenetic analysis was done by BM culture on the slide (known as karyotyping technique) for each patient. BM samples were cultured in RPMI-1640 medium (Gibco, Carlsbad, CA, USA) containing 2 mmol/l glutamine, 25 mmol/l HEPES, 1.5 g/l sodium bicarbonate, 10% fetal calf serum, 50 U/ml penicillin, and 50 g/ml streptomycin at 37°C in 5% CO2. The chromosomes obtained were then stained by Giemsa stain and later denaturalized by trypsin. Then, mitosis analysis was done for detecting the types of numerical and structural chromosome aberrations by microscopic examination.
Molecular genetic analysis was done using reverse transcription-polymerase chain reaction technique. In this regard, RNA extraction, cDNA synthesis, and PCR amplification were performed on BM samples according to the guidelines of kit manufacturer for each patient.
This study was an epidemiological analysis. Descriptive data analysis was conducted using SPSS software, version 22. Chi-square and independent samples t-test were used to assess the correlation between variables, and P < 0.05 was considered statistically significant.
| Results|| |
Sixty-five cases (90.3%) out of ALL patients, were preB lineage (42 males (64.4%) and 23 females (35.4%)). Seven cases (9.7%) were T-lineage (four males [57.1%] and three females [42.9%]).
Cytogenetic and molecular analysis
Forty-four (625) and 5 (71.4%) of patients were normal karyotype in preB and T-ALL, respectively. Translocation t(9.22) BCR-ABL (p 190) (28.6%) was seen in the remained patients of T-ALL [Table 1] and [Table 2]. T(9,22) BCR-ABL (p190) (7%) showed the highest frequency of cytogenetic and molecular genetic abnormalities in patients with preB-ALL. Three patients showed translocation of t(1.11) (4.2%) and three showed translocation of t(4.11) (4.2%). Several translocations were also observed in preB ALL patients [Table 3]. Several new genetic changes such as t(4.3) (1.4%) and inv(16) (1.4%) were observed in preB-ALL patients [Table 3] and [Table 4].
|Table 1: Frequency of cytogenetic abnormalities in seven T-acute lymphoblastic leukemia patients|
Click here to view
|Table 2: Frequency of cytogenetic abnormalities in seven T-acute lymphoblastic leukemia patients|
Click here to view
|Table 3: Frequency of molecular cytogenetic abnormalities in 65 preB acute lymphoblastic leukemia patients|
Click here to view
|Table 4: Frequency of cytogenetic abnormalities in 65 preB acute lymphoblastic leukemia patients|
Click here to view
Association between age and white blood cell with cytogenetic analysis
Translocations such as BCL11A-MYC, KMT2A-MLLT1, and TEL-AML1 seem to increase WBC count, they are not statistically significant due to the low frequency of these translocations in patients. However, there was no significant correlation between WBC count and molecular genetic disorders in patients [Table 5] and [Table 6]. Furthermore, no significant relationship was found between gender and molecular genetic abnormalities in patients [Table 7].
|Table 5: Frequency of white blood cell and molecular genetic abnormalities in 72 acute lymphoblastic leukemia patients|
Click here to view
|Table 6: Correlation between white blood cell and molecular genetic abnormalities in 72 acute lymphoblastic leukemia patients|
Click here to view
|Table 7: Correlation between gender and molecular genetic abnormalities in 72 acute lymphoblastic leukemia patients|
Click here to view
| Discussion|| |
ALL is a hematological malignancy develops as a result of the increase of the proliferation of lymphoid precursors and impairment of their differentiation. A series of genetic and molecular changes that are associated with certain clinical features cause this impairment.,, Studies showed the frequency of genetic and molecular changes in different parts of the world is different., Forestier et al. in 2000, showed that translocations of 11q23 (3.7%) and t(9; 22) (q34; q11) (2.2%) had the highest frequency in their study population. However, Andreasson et al. in 2000 showed that del (9p) was the most common chromosomal translocation in children with preB ALL. In this study, abnormal BCR-ABL (p 190) showed the highest percentage of molecular genetic abnormalities in patients with preB ALL and T-ALL [Table 2] and [Table 3]. These results are similar to the results of De Braekeleer et al. study, which showed that t (9; 22) was the most common chromosomal translocation in preB and B-ALL. Chopra et al. study also showed that t(9; 22) had the highest frequency in pediatric and adult B-ALL. In the second finding of this study is the low frequency of translocations of t(1.19), t(11.19), and t(12.21) among ALL-B ALL patients [Table 4]. This finding is in accordance with the study of Chebihi et al. the frequency of translocations of t(1.19), t(11.19), and t(12.21) was very low in B-ALL patients in this study.
Schneider et al. assessed cytogenetic abnormalities in children with T-ALL, and the relationship with the prognosis in patients and indicated t(11; 14) (7%) as the most common disorder in these patients, which was associated with a favorable prognosis. Furthermore, Chang et al. assessed cytogenetic abnormalities in children with ALL and found that t(7;14) was the most common disorder in T-ALL patients. However, in the present study, t(9,22) and BCR-ABL had the highest rate of cytogenetic abnormalities in T-ALL. In our study, several new disorders, including t(4,3) (1.4%) and inv (16) (1.4%), were introduced in addition to common cytogenetic abnormalities in preB ALL patients, which were not reported in previous studies.
Studies have shown that several characteristics such as cell count, including WBC, age, along with the identification of genetic and molecular changes, can be useful in estimating therapeutic response survival time., Awan et al., showed the increase in age, enhance the incidence of BCR/ABL in ALL patients. Patients with BCR/ABL translocation increased the count of WBC, which was associated with therapeutic resistance. Soszynska et al. found that patients in younger age had better survival and prognosis and patients with high WBC and BCR/ABL translocation were more resistant to conventional treatments and had a poor prognosis. However, in our study, no significant relationship was found between molecular genetic abnormalities and gender, and WBC counts in both ALL subtypes [Table 5], [Table 6], [Table 7].
| Conclusion|| |
Finally, we can say since genetic and molecular variations were different in compare to other studies, we concluded environmental, geographic, and population factors caused these differences among different populations. On the other hand, the evaluation of these molecular changes can be used as diagnostic and prognostic factors along with other clinical parameters in monitoring the patient and this hypothesis requires further studies in the future.
This article is issued from thesis of Yousef Tavakolifar, Pediatric Hematology and Oncology Graduate. This work was financially supported by grant TH95/5 from vice chancellor for research affairs of Ahvaz Jundishapur University of Medical Sciences.
Financial support and sponsorship
This study was funded by grant TH95/5 from vice chancellor for research affairs of Ahvaz Jundishapur University of Medical Sciences.
Conflicts of interest
There are no conflicts of interest.
| References|| |
Horowitz NA, Akasha D, Rowe JM. Advances in the genetics of acute lymphoblastic leukemia in adults and the potential clinical implications. Expert Rev Hematol 2018;11:781-91.
Paul S, Kantarjian H, Jabbour EJ. Adult acute lymphoblastic leukemia. Mayo Clin Proc 2016;91:1645-66.
Deris Zayeri Z, Tahmasebi Birgani M, Mohammadi Asl J, Kashipazha D, Hajjari M. A novel infram deletion in MSH6 gene in glioma: Conversation on MSH6 mutations in brain tumors. J Cell Physiol 2018;1-11.
Whitlock JA. Down syndrome and acute lymphoblastic leukaemia. Br J Haematol 2006;135:595-602.
Hasle H. Pattern of malignant disorders in individuals with down's syndrome. Lancet Oncol 2001;2:429-36.
Burmeister T, Schwartz S, Bartram CR, Gökbuget N, Hoelzer D, Thiel E. Patients' age and BCR-ABL frequency in adult B-precursor ALL: A retrospective analysis from the GMALL study group. Blood 2008;112:918-9.
Asnafi AA, Deris Zayeri Z, Shahrabi S, Zibara K, Vosughi T. Chronic myeloid leukemia with complex karyotypes: Prognosis and therapeutic approaches. J Cell Physiol 2018;1-9.
Mi JQ, Wang X, Yao Y, Lu HJ, Jiang XX, Zhou JF, et al.
Newly diagnosed acute lymphoblastic leukemia in China (II): Prognosis related to genetic abnormalities in a series of 1091 cases. Leukemia 2012;26:1507-16.
Yiallouros DB, Henze G. Akute lymphoblastische Leukämie (ALL). Therapie 2006;5(2006).
Guo Y, Shan Q, Gong Y, Lin J, Yang X, Zhou R, et al.
Oridonin in combination with imatinib exerts synergetic anti-leukemia effect in ph+acute lymphoblastic leukemia cells in vitro
by inhibiting activation of LYN/mTOR signaling pathway. Cancer Biol Ther 2012;13:1244-54.
Dombret H, Gabert J, Boiron JM, Rigal-Huguet F, Blaise D, Thomas X, et al.
Outcome of treatment in adults with philadelphia chromosome-positive acute lymphoblastic leukemia – Results of the prospective multicenter LALA-94 trial. Blood 2002;100:2357-66.
Jones LK, Saha V. Philadelphia positive acute lymphoblastic leukaemia of childhood. Br J Haematol 2005;130:489-500.
Gustafsson G, Schmiegelow K, Forestier E, Clausen N, Glomstein A, Jonmundsson G, et al.
Improving outcome through two decades in childhood ALL in the nordic countries: The impact of high-dose methotrexate in the reduction of CNS irradiation. Nordic society of Pediatric haematology and oncology (NOPHO). Leukemia 2000;14:2267-75.
Lawrie CH. MicroRNAs in hematological malignancies. Blood Rev 2013;27:143-54.
Schotte D, De Menezes RX, Akbari Moqadam F, Khankahdani LM, Lange-Turenhout E, Chen C, et al.
MicroRNA characterize genetic diversity and drug resistance in pediatric acute lymphoblastic leukemia. Haematologica 2011;96:703-11.
Rasras S, Zibara K, Vosughi T, Zayeri ZD. Genetics and epigenetics of glioblastoma: Therapeutic challenges. Clin Cancer Invest J 2018;7:43.
Treviño LR, Yang W, French D, Hunger SP, Carroll WL, Devidas M, et al.
Germline genomic variants associated with childhood acute lymphoblastic leukemia. Nat Genet 2009;41:1001-5.
Xu H, Cheng C, Devidas M, Pei D, Fan Y, Yang W, et al.
ARID5B genetic polymorphisms contribute to racial disparities in the incidence and treatment outcome of childhood acute lymphoblastic leukemia. J Clin Oncol 2012;30:751-7.
Forestier E, Johansson B, Borgström G, Kerndrup G, Johansson J, Heim S, et al.
Cytogenetic findings in a population-based series of 787 childhood acute lymphoblastic leukemias from the nordic countries. The NOPHO leukemia cytogenetic study group. Eur J Haematol 2000;64:194-200.
Andreasson P, Höglund M, Békássy AN, Garwicz S, Heldrup J, Mitelman F, et al.
Cytogenetic and FISH studies of a single center consecutive series of 152 childhood acute lymphoblastic leukemias. Eur J Haematol 2000;65:40-51.
De Braekeleer E, Basinko A, Douet-Guilbert N, Morel F, Le Bris MJ, Berthou C, et al.
Cytogenetics in pre-B and B-cell acute lymphoblastic leukemia: A study of 208 patients diagnosed between 1981 and 2008. Cancer Genet Cytogenet 2010;200:8-15.
Chopra A, Soni S, Verma D, Kumar D, Dwivedi R, Vishwanathan A, et al.
Prevalence of common fusion transcripts in acute lymphoblastic leukemia: A report of 304 cases. Asia Pac J Clin Oncol 2015;11:293-8.
Chebihi ZT, Belkhayat A, Chadli E, Hilal L, Skhoun H, Hessissen L, et al.
Cytogenetic profile of Moroccan pediatric acute lymphoblastic leukemia: Analysis of 155 cases with a review of the literature. Clin Lymphoma Myeloma Leuk 2018;18:e241-8.
Schneider NR, Carroll AJ, Shuster JJ, Pullen DJ, Link MP, Borowitz MJ, et al.
New recurring cytogenetic abnormalities and association of blast cell karyotypes with prognosis in childhood T-cell acute lymphoblastic leukemia: A pediatric oncology group report of 343 cases. Blood 2000;96:2543-9.
Chang HH, Lu MY, Jou ST, Lin KH, Tien HF, Lin DT, et al.
Cytogenetics in childhood acute lymphoblastic leukemia in Taiwan: A single-institutional experience. Pediatr Hematol Oncol 2006;23:495-506.
Carroll WL, Bhojwani D, Min DJ, Raetz E, Relling M, Davies S, et al
. Pediatric acute lymphoblastic leukemia. ASH Educ Program B 2003;2003:102-31.
Hoelzer D, Gökbuget N, Ottmann O, Pui CH, Relling MV, Appelbaum FR, et al
. Acute lymphoblastic leukemia. ASH Educ Program B 2002;2002:162-92.
Awan T, Iqbal Z, Aleem A, Sabir N, Absar M, Rasool M, et al.
Five most common prognostically important fusion oncogenes are detected in the majority of Pakistani pediatric acute lymphoblastic leukemia patients and are strongly associated with disease biology and treatment outcome. Asian Pac J Cancer Prev 2012;13:5469-75.
Soszynska K, Mucha B, Debski R, Skonieczka K, Duszenko E, Koltan A, et al.
The application of conventional cytogenetics, FISH, and RT-PCR to detect genetic changes in 70 children with ALL. Ann Hematol 2008;87:991-1002.
[Table 1], [Table 2], [Table 3], [Table 4], [Table 5], [Table 6], [Table 7]