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ORIGINAL ARTICLE
Year : 2015  |  Volume : 4  |  Issue : 5  |  Page : 627-632

Reduction in the numbers of CD33+ myeloid population in Egyptian children with B-linage acute lymphoblastic leukemia and its recovery after induction of chemotherapy


1 Center of Excellence in Cancer Research, Tanta University Educational Hospital; Immunology and Biotechnology Division, Department of Zoology, Faculty of Science, Tanta, Egypt
2 Center of Excellence in Cancer Research, Tanta University Educational Hospital; Hematology and Oncology Unit, Department of Pediatric, Faculty of Medicine, Tanta, Egypt
3 Immunology and Biotechnology Division, Department of Zoology, Faculty of Science, Tanta University, Tanta, Egypt
4 Center of Excellence in Cancer Research, Tanta University Educational Hospital; Clinical Pathology, Faculty of Medicine, Medical Campus, Tanta University, Tanta, Egypt
5 Center of Excellence in Cancer Research, Tanta University Educational Hospital, Tanta, Egypt

Correspondence Address:
Mohamed Labib Salem
Center of Excellence in Cancer Research, Tanta University Educational Hospital, Tanta, Egypt.
Egypt
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Source of Support: This work has been supported by a grant (ID# 5245) funded from the Science and Technology Development Fund, Ministry of Scientific Research, Egypt., Conflict of Interest: There are no conflicts of interest.


DOI: 10.4103/2278-0513.164716

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Background: Acute lymphoblastic leukemia (ALL) is biologically and clinically considered as a heterogeneous neoplasm of lymphoid progenitor cells. About 85% of children with ALL are diagnosed as B-ALL, expressing CD19; the typical marker of normal B cells. Problem: Given that the chemotherapy associated with leucopenia, in particular myeloid cells (CD33+ cells), Aim: the main aim of this study was to analyze the numbers of these cells in children with B-ALL before and after induction of chemotherapy. Materials and Methods: The frequencies of CD33+ myeloid cells and CD19+ B-cells were analyzed in the peripheral blood patients before (n = 10) and after (n = 10) induction of chemotherapy as well as in healthy volunteers (n = 10) using multiparametric flow cytometry. Results: As expected, B-ALL patients showed high numbers of CD19+ cells before induction of chemotherapy; where the numbers of these cells were reduced upon the induction of chemotherapy. CD33+ myeloid cells showed decrease in numbers in B-ALL patients before chemotherapy as compared to healthy control volunteers. Interestingly, treatment of B-ALL patients with chemotherapy-induced almost recovery of the numbers of these cells. Conclusion: CD33+ myeloid cells are increased in numbers after induction of chemotherapy, indicating to a dynamic mobilization or differentiation of their precursors into circulation. This study opens a new avenue to characterize the phenotype and function of these cells in different hematological malignancies; in particular, they may harbor regulatory cells.


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