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ORIGINAL ARTICLE
Year : 2015  |  Volume : 4  |  Issue : 5  |  Page : 603-609

Children with acute lymphoblastic leukemia show high numbers of CD4+ and CD8+ T-cells which are reduced by conventional chemotherapy


1 Center of Excellence in Cancer Research, Tanta University Educational Hospital; Immunology and Biotechnology Division, Zoology Department, Faculty of Science, Tanta University, Tanta, Egypt
2 Center of Excellence in Cancer Research, Tanta University Educational Hospital; Hematology/Oncology Unit, Pediatric Department, Faculty of Medicine, Tanta University, Tanta, Egypt
3 Histology and Histochemistry Division, Zoology Department, Faculty of Science, Tanta University,Tanta, Egypt
4 Center of Excellence in Cancer Research, Tanta University Educational Hospital; Clinical Pathology, Faculty of Medicine, Medical Campus, Tanta University, Tanta, Egypt
5 Center of Excellence in Cancer Research, Tanta University Educational Hospital, Tanta, Egypt

Correspondence Address:
Mohamed Labib Salem
Center of Excellence in Cancer Research, Tanta University Educational Hospital, Tanta, Egypt.
Egypt
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Source of Support: This work has been supported by a grant (ID number 5245) funded from the Science and Technology Development Fund, Ministry of Scientific Research, Egypt to Mohamed L. Salem, the Principal investigator of this project., Conflict of Interest: There are no conflicts of interest.


DOI: 10.4103/2278-0513.164717

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Background: Acute lymphoblastic leukemia (ALL) is considered as one of the most common cancer in pediatric malignancies. Among ALL, B-cell Acute Lymphoblastic Leukemia (B-ALL) represents 80% to 85% of the childhood ALL. Problem: Although anti B-ALL chemotherapy kill B-ALL, it associates with alteration in the numbers of CD4+ and CD8+ T-cells, and thus impacts the overall immunity. Aim: To evaluate the impact of anti B-ALL on the numbers of CD4+ and CD8+ T-cells in correlation to the numbers of CD10+ B cells in B-ALL pediatric patients. Materials and Methods: Peripheral blood samples were drawn from previously diagnosed B-ALL before (n = 10 cases) and after (n = 10 cases) chemotherapy as well as from healthy controls (n = 10 cases). The numbers of CD4+, CD8+ T-cells and CD10+ B cells were measured in these samples by flow cytometry. Results: As expected, the numbers of CD10+ B-cells were increased in B-ALL patients before chemotherapy which were associated with increases in the numbers of CD4+ and CD8+ T-cells. Chemotherapy of B-ALL patients, during the induction phase, induced dramatic decreases in the numbers of CD10+ B cells, which were associated with decreases in the numbers of CD4+ and CD8+ T-cells. Tin spite of this alteration, the ratio of CD4/CD8 in B-ALL patients were remained similar before and after chemotherapy as compared to those in healthy controls. Conclusion: Anti B-ALL chemotherapy induces alterations in the frequencies of T-cell subsets. Given the importance of these cells in anti-tumor immunity, our data may lead to further studies to investigate the different subsets of these cells, in particular regulatory T-cells.


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